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Innovative rapid solid-phase immunobead assay for the detection of okadaic acid and related DSP toxins in shellfish ArchiMer
Park, D; Fremy, J; Marcaillou, L; Gamboa, P; Gleizes, E; Masselin, P; Goldsmith, C.
The solid-phase immunobead assay (Ciguatect super(TM)) has been optimized to detect toxins associated with diarrheic shellfish poisoning (DSP). The Ciguatect super(TM) test kit utilizes a membrane to bind the toxins and a monoclonal antibody-latex bead solution as the determination step. The presence or absence of okadaic acid (OA) and related toxins is determined by binding the toxins to a membrane attached to a plastic strip and exposing the toxins ladened membrane to a monoclonal antibody-colored latex bead complex which has a high specificity for OA and related polyether compounds. The intensity of the color on the membrane denotes the presence of the toxins. A rapid extraction method (REM super(TM)) has been developed to allow for confirmation of...
Tipo: Text Palavras-chave: Dinophysis; Bivalvia; Algae; Marine molluscs; Immunology; Bioassays; Disease detection; Biological poisons; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1604.pdf
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The use of API 20E for the identification of <i>Vibrio anguillarum</i> and <i>V. ordalii</i> OMA
Grisez, L.; Ceusters, R.; Ollevier, F.P..
Sixty-eight strains of <i>Vibrio anguillarum</i>, five of <i>V. ordalii</i> and the type strains of <i>V. alginolyticus, V. carchariae, V. damsela</i> and <i>V. parahaemolyticus</i> were compared using the API 20E gallery. Within the <i>V. anguillarum</i> strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. <i>Vibrio ordalii</i>, the former <i>V. anguillarum</i> biotope 2, could easily be separated from <i>V. anguillarum</i> and from the other fish pathogenic <i>Vibrio</i> spp.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859
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